Flo was created by breeder DJ Short in the 1990s when he crossed a hybrid of Highland Thai with Purple Thai (a cross of Chocolate Thai with Oaxacan Gold,) with an Afghani landrace strain.

There are two main Flo phenotypes, one with light-green coloring, and the other selected for its blue pigmentation.

Consumers savor Flo for its menthol and hash flavor, that stems from its high levels of linalool and alpha-pinene.

Cannabis Canada Magazine named Flo the #1 strain at the 1996 Cannabis Cup.